The role of wild deer in the transmission of diseases of livestock

Betaretrovirus and Picobirnavirus has now been detected in wild fallow, rusa, sambar and chital deer. This virus has been considered an opportunistic gastrointestinal pathogen, however, reports of its detection in extra-intestinal sites have raised a debate regarding their actual site of infection. Respiratory tract samples are currently being screened.

The project is revealing that the risk posed by deer as hosts of exotic disease is real. Analysis has revealed the presence of novel Betaretrovirus and Picobirnavirus in serum samples from wild fallow, rusa, sambar and chital deer. The extent of the risk and likelihood of transference of these and other diseases from deer to livestock is subject to further investigation.
Screening is currently underway to determine the prevalence of these novel viruses in deer and livestock populations across Australia.

The majority of the laboratory work for the disease screening of the deer samples has been completed, while population genetics is still ongoing.
Sequences from two putative RNA viruses have been identified and completion of sequencing data and formal description is ongoing.
Two different strains of a gastro-intestinal protozoa have also been identified. Both are known to be able to infect livestock species and may have an impact for the industry.

A high-throughput sequencing system (Illumina Hiseq) was used to analyse five serum samples from wild Fallow deer. The sequencing depth was increased five-fold from initial sequencing analysis to provide a greater scope of detection. Preliminary evidence of viral genetic material was found in some samples with confirmation and further analysis ongoing.
Microscopic examination of blood smears from NSW has revealed the presence of several morphological forms, possibly compatible with blood-protozoa. DNA from each blood sample was tested for seven parasitic genera using PCR, returning a negative result for these parasite groups. Analysis of additional blood samples is ongoing.
Deeper analysis of the viral sequences found in the last trial of metagenomics is necessary. This will allow the development of specific primers to detect individual viruses using PCR. PCR analysis of the blood samples from NSW revealed negative results, despite microscopic analysis revealing evidence of possible presence of some microorganisms. Metagenomics analysis of a specific RNA region will help identify the microorganism and provide information to develop a screening tool.

Faecal, blood and DNA samples have been collected from deer species across Australia and analysis undertaken to confirm species.

Serum (blood) samples were screened for the presence of antigens and antibodies against Pestivirus and Bovine herpesvirus-1 using commercial serological tests. Antigens and antibodies against Pestivirus were detected in a very small number of the samples, while no serological evidence of Bovine herpesvirus-1 were detected in the samples.

For a subset of the blood samples collected, blood smears were performed on site at the time of blood collection. Microscopy examination of 50 blood smears revealed the presence of several morphological forms, possibly compatible with blood-protozoan. The corresponding blood samples were screened by PCR for known blood parasites. Published primers targeting conserved regions of seven parasitic genera commonly infecting livestock species were selected for molecular identification. PCR conditions and protocols were optimised to enable large scale screening, and analysis is currently ongoing.