Rabbit biocontrol has had dramatic and widespread benefits to Australian agriculture and environment for 60 years, with benefits estimated at over $70 billion. For 20 years only one type of virulent RHDV has been circulating in Australia, to which some populations of rabbits started to develop genetic resistance. Since 2014, with the detection of two exotic virulent RHDVs, and the coordinated release of the RHDV1 K5 strain, there are now four virulent strains of RHDV present in Australia’s rabbit population. This increasing genetic diversity poses challenges as well as opportunities.
Unfortunately, there is never likely to be a silver bullet in rabbit management. We know from monitoring studies that the best response is an ongoing one, ensuring that new rabbit biocontrol agents can be released on a regular basis to counteract reduced effectiveness of existing agents due to increasing immunity and genetic resistance. In addition, boosts to existing controls add to their effectiveness, particularly through a horses-for-courses type approach. As such, rabbit management is not about one-off applications of solutions but regular, community-based approaches drawing from a pipeline of new, existing and evolving solutions.
Completed
A molecular virologist by training, Tanja’s research over the past 15 years has focused on mitigating the impacts of invasive vertebrate pest species in Australia, using both lethal and non-lethal, GM and non-GM methods, targeting a series of species from Red foxes to mice, cane toads and in particular rabbits. Her earlier work with CSIRO investigated the development of virally vectored immunocontraception vaccines for foxes and mice, for which she was awarded the Pest Animal Control CRC Chairman’s medal for scientific excellence in 2004 and 2008.
During the past ten years Tanja has driven a combination of applied and basic fundamental research projects focussing on improving our understanding of the diseases used for classical biological control of rabbits. She has built an international track record in the area of rabbit calicivirus biology, ecology, epidemiology, evolution and virulence, which is evidenced in a series of high quality publications and invitations to present at national and international conferences.
Tanja has a PhD in molecular virology from Philipps Universitaet Marburg, Germany
The project receives funding from the Australian Government Department of Agriculture, Water and the Environment
Note: If you are a vet or domestic rabbit pet owner you can now submit samples directly for testing via this CSIRO portal – https://research.csiro.au/rhdv/ (follow the prompts)
February 2021 update:
Community submission of rabbit samples continues to deliver valuable insights into the changing epidemiology of the various circulating virus strains in Australia. Derivatives of the original Australian RHDV2 continues to be dominant in WA, while various recombinant RHDV2 variants are dominant throughout the Eastern states.
Analysis of sequence data provided strong evidence of RHDV1 activity inhibition as a result of initial RHDV2 spread, strengthening previous evidence derived from serological data that RHDV2 suppressed the impact of RHDV1-K5 when it was released in 2017.
August 2020 update:
RHDV2 and its recombinants continue to be epidemiologically dominant in the Australian landscape, however we are seeing growing evidence of some local persistence and spread of RHDV1-K5 in Western Australia and Tasmania, and the detection of an RHDV1 field strain demonstrates that this original virus variant has not yet become completely extinct.
RHDV2 causes virus outbreaks and transmission to occur approximately one season earlier than RHDV1. The ability to infect younger rabbits may be a key competitive advantage for RHDV2 in the field, consequently altering the epidemiology of the virus in wild populations of rabbits.
February 2020 update:
While RHDV2 remains the dominant virus circulating in the field in all areas of Australia, several cases of RHDV1 K5 have been detected one location that were not associated with a targeted release. These results suggest that RHDV1 K5 may have become established in wild rabbit populations in some locations. Further monitoring will be carried out through the project to better understand if this has occurred more widely or remains the exception.
CSIRO has developed a new assay for high throughput screening of recombinant caliciviruses. This assay has now been adopted into the routine testing protocols for RabbitScan and has retrospectively been applied to all positive calicivirus samples to confirm the identity of each sample.
A pilot study investigating the suitability of using carrion feeding flies as a better broad scale tool to monitor disease activity at a landscape scale is nearing completion of its first year. Preliminary analysis of the first nine months of samples (March 2019 to January 2020) indicate that RHDV activity can indeed be detected in fly samples, but the sensitivity of this method remains unclear at this stage. The aim is to continue this study for an additional 12 months to get a better understanding of this tool to monitor RHDV activity at a wider scale.
August 2019 update:
Ongoing testing of tissue from deceased rabbits shows RHDV2 remains the dominant rabbit calicivirus circulating in Australia, with K5 detected at release sites. A new screening method is being implemented to detect recombinant viruses.
Rabbit monitoring at selected intensive sites in TAS, ACT, WA, NSW and SA is ongoing or ramping up shortly, with a minimum bi-annual sampling taking place. In addition, monitoring and sampling of the rabbit population at the Turretfield research site continues as part of this project, building on extensive knowledge of this wild rabbit population and adding these ongoing monitoring results to the long-term RHDV and myxoma disease database at this site.
A paper on optimising and validating molecular testing of carrion flies for rabbit calicivirus detection, including associated protocols was published. This provided the basis for a nationwide year-long pilot study now underway.
February 2019 update:
Intensive rabbit monitoring sites have been established with monitoring well underway at sites in South Australia and the ACT, and due to commence at other sites in Autumn 2019. Sites in South Australia are reporting the lowest abundance of rabbits in more than 20 years, indicating that circulating strains of RHDV are suppressing rabbit numbers.
The methods for sampling, processing and analysing carrion feeding flies for the presence of RHDV have been developed and validated. A national fly monitoring network at over 30 sites across Australia has been established and fortnightly sampling has commenced.
Analysis of samples from dead rabbits submitted directly to CSIRO and through RabbitScan is ongoing – over 1600 samples have been analysed since the beginning of the previous IA-CRC project RHD Boost Rollout, and ~1000 since the K5 release).
Reports:
Scientific publications: